opium induces apoptosis in jurkat cells via promotion of pro-apoptotic and inhibition of anti-apoptotic molecules
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abstract
objective(s): the aim of this study was to determine the important molecules involved in apoptosis induction by opium in jurkat cell line. materials and methods: jurkat cells were incubated 48 hrs with2.86×10-5 g/ml concentration of opium and apoptosis as well as expression levels of related molecules weremeasured. results: our results demonstrated that 50.3±0.2 percent of opium treated jurkat cells were revealed apoptotic features. the levels of mrna of several pro-apoptotic and anti-apoptotic molecules were increased and decreased, respectively, in the opium treated cells. the results also demonstrated that expression levels of bcl2, dffa and nol3 as anti-apoptotic molecules were increased in the opium treated cells. conclusion: it seems that opium induces apoptosis in jurkat cells via both intrinsic and extrinsic pathways. although opium induces apoptosis in the cells but increased expression of some anti-apoptotic molecules may be a normal resistance of the cell for death.
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Opium induces apoptosis in Jurkat cells via promotion of pro-apoptotic and inhibition of anti-apoptotic molecules
Objective(s): The aim of this study was to determine the important molecules involved in apoptosis induction by opium in Jurkat cell line. Materials and Methods: Jurkat cells were incubated 48 hrs with2.86×10-5 g/ml concentration of opium and apoptosis as well as expression levels of related molecules weremeasured. Results: Our results demonstrated that 50.3±0.2 percent of opium treated Jurka...
full textOpium Induces Apoptosis in Jurkat Cells
Background: The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. Methods: Different concentrations of opium (2.86 × 10-3 to 2.86 × 10-11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. Apoptotic events were assessed after 6, 24, and 72 hours by flow-cytometric...
full textOpium Induces Apoptosis in Jurkat Cells
BACKGROUND The direct effect of some opioids on immune cells has been demonstrated. The aim of this study was to assess the apoptotic effect of opium on Jurkat T lymphocyte cells. METHODS Different concentrations of opium (2.86 × 10-3 to 2.86 × 10-11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. Apoptotic events were assessed after 6, 24, and 72 hours by flow-cytometric ...
full textIranian Journal of Basic Medical Sciences
Article type: Short communication Objective(s): The aim of this study was to determine the important molecules involved in apoptosis induction by opium in Jurkat cell line. Materials and Methods: Jurkat cells were incubated 48 hrs with 2.86×10-5 g/ml concentration of opium and apoptosis as well as expression levels of related molecules were measured. Results: Our results demonstrated that 50.3±...
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Journal title:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۲، صفحات ۲۱۵-۲۲۰
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